Tuesday, April 30, 2013

Microscope


In order to focus on an item in high power, you must start with the scanning objective and then transition from low to high. For the scanning and low objectives, you can use the fine and coarse adjustment knobs as needed, but when focusing on an item in high power, use the fine adjustment knob only; the coarse adjustment knob can cause scratching.

To make a wet mount, place the specimen on the middle of the slide, making sure it isn't too thick to be observed. Then, place one drop of water onto the specimen and place the cover slip at a 45 degree angle with one edge touching the water drop, gently letting it go.

To stain a specimen, you must first prepare it using the same procedure as a wet mount. Then, place one drop of dye on the edge of the cover slip. Place the edge of a paper towel on the opposite of the cover slip; it will draw the water from under the cover slip and pull the dye onto the specimen.

Though this was not my first time using a microscope, it was definitely helpful to have an opportunity to review. I did not remember every single detail about microscopes since I have not used them frequently in a while, but the microscope lab helped refresh my memory.

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